Hemoglobin A0 is prepared from lysed human blood cells by sequential ion-exchange. It is a mixture of non-glycated and glycated hemoglobin A0 fractions, but is essentially free of HbA1a, HbA1b, HbA1c and HbF. The protein is valedated by HPIEC methods, and is suitable as a primary standard in chromatographic or immunologic analyses. The reported concentration is determined by Drabkins assay.
The material is supplied in liquid form in dilute potassium cyanide-phosphate buffer. The concentration varies with manufacturing lot, but the protein may be used directly by dilution in buffer in most experimental methods. It is shipped frozen at -20oC
Short term storage should be done at 4oC. Long term storage should be completed at -60oC. Repeated cycles of freezing and thawing should be avoided.