|h Glycated Albumin is a protein purified by sequential ion-exchange and affinity chromatography. NGA is albumin that is glycated in vivo; it is isolated from human plasma. SGA is albumin that glycated in vitro, after short-term incubation with glucose, and subsequently purified. The preparations are pure by SDS-PAGE and agarose gel electrophoresis, and have 0.3-1.5 glyco-groups per mole of albumin. NGA and SGA are immunoreactive in microplate based ELISAs and in Western Blots designed to detect albumin or glycoalbumin. When diluted appropriately, h glycated Albumin shows a dose-response identical to that of fresh human plasma in Glycaben (Exocell Catalogue #1003). The material is suitable for use as a primary standard in immunoassay methods.
HSA, a related product, is deglycated-albumin. It serves as a negative control in immunochemical procedure's that detect glycoalbumin.
These products are supplied in liquid or lyophilized forms.
Short term storage should be done at 4oC. Long term storage should be completed at -60oC. Repeated cycles of freezing and thawing should be avoided.