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Apolipoprotein B


Manufacturer/Trade: Exocell / Apo B Test
Catalogue Numbers: 1006 Strip Plate
Methodology: Indirect Competitive ELISA
Summary of procedure: The Apo B Test is designed for the quantitative measurement of apolipoprotein B in human plasma. To complete the assay, B36 monoclonal antibody is added to a microtiter plate well that contains apolipoprotein B immobilized to the solid phase, and to which apolipoprotein B, standard or sample, is added in the fluid phase. During a 30 minute incubation, the monoclonal antibody binds to either the immobilized apo B or to the soluble apo B, hence the notion of competitive binding. Unbound reactants are then washed away, and a second incubation of 15 minutes with an enzyme conjugated to an anti-mouse antibody reacts with the B36 that was captured by the solid phase. The wells are washed to remove unbound secondary antibody, and TMB Substrate solution is added to detect bound conjugate. The chromogenic reaction is stopped by an addition of dilute acid, and color intensity determined by absorbance at 450nm. Sample concentration is determined by relating response to that for known standards.
Specimen Required: 10ul citrate or EDTA treated plasma
Assay Range: 0.13-2.0 mg/ml (corresponding to 13-200mg/dl)
Precision: Intra- and interassay precision of samples within the assay range have a C.V.<10%.
Specificity: The monoclonal antibody B36 is specific for human apolipoprotein B, and does not cross-react with other proteins or substances found in human plasma.
Expected Values: Expected values for apo B in male subjects are reported to be 108 +/- 33 mg/dl for individuals free of coronary artery disease (CAD), and 131 +/- 37 for subjects with angiographically documented premature CAD. Values for women are similar to those for men.



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