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r Glycated Albumin


Manufacturer/Trade: Exocell / Glycabumin
Catalogue Numbers: GAR-50
50 determination tube assay
Methodology: Sandwhich ELISA
Summary of procedure: Glycabumin is an enzyme linked immunosorbent assay for the quantitative determination of glycoalbumin in rat plasma. It provides information concerning glycemic control analogous to that offered by glycohemoglobin measurements, except that the protein measured is glycated albumin which has a shorter half-life.

The assay depends upon a monoclonal antibody, A717, that specifically recognizes the glycated moieties in glycated albumin. In practice, standards or samples are added to A717 coated tubes. The r glycoalbumin in these analytes binds to or is captured by the antibody on the solid phase, and unbound materials are washed away. A subsequent incubation with an enzyme conjugated anti-rat albumin antibody leads to detection of the bound molecules. Bound conjugate is determined colorimetrically after reaction with a TMB substrate solution.

By relating samples response with that for the standard, the concentration of rat glycoalbumin may be determined. A separate analysis of total albumin allows the results to be converted to percent total albumin that is glycated.

Specimen Required: 50ul citrate of EDTA treated plasma
Assay Range: 0.2-2 mg/ml glycated albumin
Precision: Intra- and interassay precision of samples within the assay range have a C.V. <10%.
Specificity: The monoclonal antibody A717 is specific for glycated albumin, and does not cross-react with other proteins that occur in human plasma including non-glycated albumin. The enzyme conjugated antibody preparation is specific for rat albumin.



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