| Non-radioactive immunoassay for the quantitative determination of glycated LDL in plasma to evaluate glycemic control in diabetes, and to assess the contribution of the modified protein to vascular disease. The assay depends upon ES12, a monoclonal antibody directed against a specific epitope in glycated LDL. The glycated LDL is measured in apolipoprotein B equivalents, and is expressed as ug/ml or mg/dl. Glycacor is a competitive ELISA in a microplate format.
To complete the assay, the ES12 is added to a microtiter plate that contains immobilized glycated LDL and soluble glycated LDL (standard or experimental sample). The monoclonal antibody binds to either the immobilized glycated LDL or the soluble phase glycated LDL during the primary incubation, hence the notion of competitive binding. Reactants in the fluid phase are subsequently washed away, and captured ES12 on the solid phase is detected with an enzyme conjugated anti-mouse antibody. The results are made quantitative after chromogenic reaction with TMB.