The Glyco-Albumin R assay is an ELISA designed specifically for use with rat serum samples. In practice, sera are diluted 1:11 to fall within the useful range of the assay.
The assay depends upon the binding of rat glycated albumin in standard or sample to specially treated microtiter wells. Bound analyte is detected with an anti-rat albumin antibody-HRP conjugate. After a final wash, bound conjugate is detected using a chromogenic substrate (TMB) for peroxidase, and color intensity is directly porportional to glycated albumin concentration. Typical glycated albumin values lie between 0.1 and 0.6 mg/dL, abut normal values may vary with different stranis of rats.