Manufacturer/Trade: Exocell/CRP ELISA
Catalogue Numbers: 1022
Methodology: Quantitative ELISA
Summary of Procedure:

The CRP-ELISA is designed to measure C-reactive protein in rodent and other animal models of disease and is applicable to multiple species. C-reactive protein (CRP) is an acute-phase protein produced in the liver following tissue damage caused by inflammation, infection, or trauma. Serum CRP levels quickly increase following acute tissue damage and fall rapidly after removal of the stimulating source, making the measurement of CRP an excellent biomarker of inflammatory conditions that would otherwise be difficult to detect and monitor.

Specimen Required: Serum or plasma.
Mouse: 100 ul of 1:10 dilution
All others: 100 ul 1:4000 dilution
Culture medium and other fluids may be adapted for the assay.
Assay Range: Mouse: 0.25 ng/ml – 25 ng/ml
All others: 1.0 ng/ml – 100 ng/ml
Precision: Intra- and interassay precision for samples within the assay range have a C.V. <7%.

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